Thiazole Orange
Thiazole Orange is a high-sensitivity, cell-permeant dye that binds DNA and RNA, greatly enhancing green fluorescence. It offers strong signals, low background, and broad compatibility specifically for nucleic acid detection in imaging, flow cytometry, molecular biology, and diagnostic assays.
Key Features and Benefits
-
High Affinity for Nucleic Acids: Strong binding to both DNA and RNA with high fluorescence enhancement.
-
Bright Green Emission: Excitation/Emission maxima around 512/533 nm for sensitive detection.
-
Cell-Permeant: Enables live-cell and fixed-cell applications.
-
Versatile Detection: Works in fluorescence microscopy, flow cytometry, and gel staining.
-
High Sensitivity: Produces strong signal with minimal background interference.
Applications
-
DNA and RNA staining in fluorescence microscopy.
-
Flow cytometry for nucleic acid content analysis.
-
Live-cell imaging and viability assays.
-
Gel and capillary electrophoresis detection.
-
Research in molecular diagnostics and cytogenetics.
Storage and Handling
-
Storage: Store at –20 °C, dry, and protected from light.
-
Handling: Prepare solutions in DMSO or aqueous buffers; avoid prolonged light exposure to preserve dye performance.
This product is a bright, cell-permeant nucleic acid dye with strong fluorescence enhancement upon binding to DNA or RNA, especially ideal for imaging, flow cytometry, and molecular detection assays.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
