TCO-PEG7-NHS ester
TCO-PEG7-NHS ester is a high-purity PEG linker with a trans-cyclooctene (TCO) for rapid, catalyst-free tetrazine ligation and an NHS ester for amine conjugation. The PEG7 spacer specifically enhances solubility and flexibility, ensuring efficient bioconjugation in aqueous and organic systems for drug delivery, diagnostics, and advanced materials.
Key Features and Benefits
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TCO Functional Group: Enables fast, catalyst-free inverse electron-demand Diels–Alder (IEDDA) click chemistry with tetrazines.
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NHS Ester: Couples efficiently with primary amines to form stable amide bonds.
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PEG7 Spacer: Improves solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Supports orthogonal conjugation strategies.
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High Purity: Supplied at >95% purity for reproducible, high-yield performance.
Applications
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Bioorthogonal Labeling: Attach TCO handles for rapid tetrazine ligation.
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Protein and Peptide Modification: Conjugate to amines while reserving TCO for click chemistry.
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Antibody–Drug Conjugates (ADCs): Enable site-specific payload attachment.
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Nanoparticle and Surface Functionalization: Add TCO groups for targeted conjugation.
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Diagnostic Probe Development: Create multifunctional imaging and sensing agents.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMSO or DMF; avoid moisture to preserve NHS ester and TCO reactivity.
TCO-PEG7-NHS ester combines rapid, catalyst-free TCO–tetrazine click chemistry with efficient amine coupling in a hydrophilic PEG7 spacer, making it an especially versatile tool for multifunctional bioconjugates and precision linker design.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
