TCO-PEG3-maleimide
This product is a compact heterobifunctional linker that combines a trans-cyclooctene (TCO) group, a short PEG3 spacer, and a maleimide functionality. The TCO enables ultrafast copper-free click chemistry with tetrazines, while the maleimide reacts selectively with thiols to form stable thioether bonds. The PEG3 spacer specifically improves solubility and reduces steric hindrance, making this reagent highly efficient for bioconjugation.
Key Features and Benefits
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TCO Group: Enables rapid, copper-free ligation with tetrazines via inverse electron-demand Diels–Alder (IEDDA) chemistry.
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Maleimide Group: Reacts selectively with thiols on cysteine residues for stable conjugation.
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PEG3 Spacer: Enhances solubility and flexibility while maintaining a compact molecular design.
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Dual Reactivity: Combines thiol-specific labeling with bioorthogonal click chemistry.
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High Purity: Manufactured to ≥95% purity for reproducible research applications.
Applications
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Bioconjugation: Couples thiol-containing biomolecules with tetrazine-functionalized partners.
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Protein and Antibody Labeling: Enables site-specific modification through cysteine residues.
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Drug Delivery: Serves as a linker in antibody–drug conjugates (ADCs) and targeted therapeutics.
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Chemical Biology: Facilitates compact orthogonal labeling strategies in complex biological systems.
Storage and Handling
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Storage: Store at –20 °C in a tightly sealed container, protected from light and moisture.
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Handling: Dissolve freshly in dry solvent before use; avoid repeated freeze–thaw cycles.
This product integrates rapid TCO click reactivity with thiol-selective maleimide chemistry, specifically providing a compact and versatile tool for protein labeling, drug delivery, and advanced bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
