TAMRA DBCO, 5-isomer (CAS: 1911598-65-6) is a derivative of tetramethylrhodamine (TMR, TAMRA) containing a cyclooctyne moiety (dibenzocyclooctyne, DBCO). This unique structure enables it to participate in strain-promoted azide-alkyne cycloaddition (SPAAC) reactions, where it reacts with azide-functionalized molecules quickly and efficiently without the need for a copper catalyst. This makes it ideal for copper-free click chemistry, especially in live-cell imaging and in vivo applications, where avoiding toxic copper is crucial.
Key Features:
- CAS Number: 1911598-65-6
- Molecular Formula: C52H54N6O8
- Fluorescent Properties: The TAMRA 5-isomer has excitation/emission maxima at approximately 553/575 nm, making it comparable to DyLight 549.
Applications:
- Bio-Conjugation: TAMRA DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. This includes labeling in environments where high-efficiency and bio-compatibility are essential.
- Live-Cell Imaging and In Vivo Labeling: The copper-free click reaction enabled by the DBCO group is particularly well-suited for live-cell applications where non-toxic conditions are required.
- Fluorescent Detection: Its bright fluorescence makes it a popular choice for techniques like fluorescence microscopy, flow cytometry, and immunofluorescence assays, providing high visibility and stability during experiments.
Advantages:
- Copper-Free Click Chemistry: The DBCO moiety allows for rapid and selective conjugation with azides without a copper catalyst, making it ideal for sensitive biological systems.
- Efficient Reaction: DBCO reacts quickly and efficiently with azide groups, streamlining the labeling process.
Storage and Handling:
- Storage: Store at -20°C in a dry, dark environment to maintain stability.
Ideal for:
- Molecular Labeling: Proteins, nucleic acids, peptides, and other biomolecules containing azide groups.
- Sensitive Biological Systems: Ideal for environments requiring non-toxic and copper-free conditions, such as live-cell imaging and in vivo labeling.
This versatile fluorescent dye provides bright, stable fluorescence while enabling non-toxic labeling through copper-free SPAAC reactions. It is particularly useful for research in molecular biology, synthetic biology, and diagnostics.
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