N-(2-(bicyclo[2.2.1]hept-5-ene-2-carboxamido)ethyl)pyrene-1-carboxamide is a chemical compound used as a fluorescent probe in biochemistry and molecular biology research. It is a derivative of pyrene-1-carboxamide, which contains a pyrene fluorophore attached to a carboxamide group, with a linker that contains a bicyclo[2.2.1]hept-5-ene-2-carboxamide group.
The bicyclo[2.2.1]hept-5-ene-2-carboxamide group is a bicyclic compound that contains a cycloheptene ring fused to a cyclopropane ring. This unique structural feature allows for increased rigidity and specificity of the probe.
N-(2-(bicyclo[2.2.1]hept-5-ene-2-carboxamido)ethyl)pyrene-1-carboxamide is often used as a fluorescent probe for studying protein-protein interactions, as the pyrene group can be excited by light and emit fluorescence in response to binding events. The compound has also been used in the development of biosensors and other analytical tools for detecting and quantifying biomolecules.
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