Linear Polyacrylamide (LPA) is a synthetic polymer used to improve nucleic acid precipitation, especially when working with low-concentration DNA or RNA samples. Unlike biological carriers like glycogen or tRNA, LPA is chemically inert and free of contaminants, ensuring high-purity recovery without interfering with downstream applications like PCR, cloning, or sequencing.
Key Benefits:
- Better Recovery: LPA enhances nucleic acid precipitation, even at very low concentrations, improving yields.
- Chemical Stability: Being synthetic, LPA is free from nucleases and other contaminants.
- Application Compatibility: LPA doesn’t affect enzymatic reactions or spectrophotometric measurements at 260 nm and 280 nm.
How to Use LPA for Precipitation:
- Add LPA: Mix 10–20 µg of LPA with the nucleic acid sample before adding alcohol (ethanol or isopropanol).
- Precipitate: Add salts like sodium acetate, incubate at a low temperature, and allow nucleic acids to aggregate.
- Centrifuge: Spin the sample to pellet the nucleic acids and LPA complex.
- Wash and Resuspend: Rinse with 70% ethanol to remove impurities, then dissolve the nucleic acids in nuclease-free water or buffer.
LPA simplifies nucleic acid precipitation by boosting recovery and maintaining sample purity, making it an essential tool in molecular biology research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
