L-Azidohomoalanine (AHA), CAS: 942518-29-8, is a methionine analog with an azido group that integrates into proteins during active synthesis in cultured cells. This azido moiety enables detection through “click” chemistry with alkyne or cyclooctyne reagents, allowing precise protein labeling.
Key Features of AHA:
- Methionine Replacement: Incorporates into proteins during synthesis.
- Azido Group: Enables selective labeling via click reactions.
- High Sensitivity: Detects proteins at low femtomole levels in gels and blots, compatible with LC-MS/MS and MALDI MS.
Applications:
- Protein Labeling: Tags proteins in live cells to study synthesis.
- Click Chemistry: Binds to fluorescent or biotin-alkyne probes for imaging and analysis.
- Proteomics: Aids in studying protein dynamics and interactions.
- Mass Spectrometry: Supports sensitive protein analysis with LC-MS/MS and MALDI MS.
AHA’s ability to tag newly synthesized proteins with high precision makes it essential for advanced protein research and detection.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
