Eosin-5-maleimide

Eosin-5-maleimide

Eosin-5-maleimide is a potent photosensitizer that efficiently labels thiol groups in proteins and peptides. It generates singlet oxygen with a high quantum yield of 0.57, making it ideal for photoconversion of electron-rich materials like DAB in electron microscopy. Moreover, it acts as a fluorescence acceptor in FRET studies with dyes like fluorescein (FAM), dansyl, and coumarin.

Key Features:

• Photosensitizer: Generates singlet oxygen (quantum yield 0.57) for DAB photoconversion.
• Thiol-Specific: Efficiently labels cysteine residues, forming stable conjugates.
• Fluorescence: Excitation/emission at 524/545 nm, with strong absorbance (ε = 100,000).

Applications:

• Protein Labeling: Labels thiols for bioconjugation in proteins and peptides.
• Electron Microscopy: Photooxidizes DAB for high-resolution studies.
• FRET: Serves as an acceptor for FRET with FAM, dansyl, and coumarin.

Advantages:

• High Efficiency: Generates singlet oxygen for precise photoconversion.
• Stable Conjugation: Forms strong, long-lasting bonds with thiol groups.
• Versatile: Works in FRET, electron microscopy, and bioconjugation.

Eosin-5-maleimide is perfect for researchers needing a fast, reliable photosensitizer for thiol labeling, FRET, and electron microscopy.

Storage: Store at -20°C. Protect from light. Soluble in DMF or DMSO.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.