endo-BCN-PEG3-Biotin
endo-BCN-PEG3-Biotin is a high-purity heterobifunctional linker with an endo-bicyclo[6.1.0]non-4-yne (endo-BCN) for rapid, copper-free SPAAC click chemistry and a biotin for high-affinity streptavidin/avidin binding. The PEG3 spacer specifically enhances solubility, flexibility, and reduces steric hindrance, ensuring efficient conjugation and optimal biotin–protein interaction. This reagent is especially ideal for bioorthogonal labeling, affinity purification, and biotin-based detection assays in aqueous and organic systems.
Key Features and Benefits
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Endo-BCN Group: Enables fast, catalyst-free SPAAC click chemistry with azides.
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Biotin Moiety: Provides strong, specific binding to streptavidin or avidin.
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PEG3 Spacer: Improves solubility and molecular flexibility, minimizing steric interference.
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Heterobifunctional Design: Supports orthogonal, site-specific modifications.
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High Purity: Supplied at >95% purity for reproducibility.
Applications
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Copper-free bioorthogonal labeling of azide-functionalized biomolecules.
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Streptavidin-based affinity capture and purification.
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Biotinylation of proteins, peptides, nucleic acids, and nanoparticles.
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Biosensor and diagnostic assay development.
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Surface functionalization for imaging and detection.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to maintain BCN reactivity.
This product combines copper-free click chemistry with strong biotin–streptavidin binding via a PEG3 spacer, specifically offering high solubility and flexibility for labeling, detection, and purification applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
