DMTR-biotin-PEG2-amine
This product is a high-purity, heterobifunctional biotinylation reagent with a DMTr-protected amine and a biotin moiety specifically linked by a PEG2 spacer. The PEG2 improves solubility and flexibility, while the DMTr group allows selective deprotection for orthogonal, precise bioconjugation and assay development.
Key Features and Benefits:
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Biotin Moiety: Enables high-affinity binding to streptavidin or avidin-based systems for detection, purification, and immobilization.
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DMTr-Protected Amine: Allows controlled deprotection for orthogonal or stepwise functionalization.
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PEG2 Spacer: Improves solubility, flexibility, and accessibility of the reactive groups.
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Heterobifunctional Design: Supports sequential conjugation strategies for complex biomolecule assembly.
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High Purity: Supplied at >95% purity for reliable and reproducible performance.
Applications:
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Biotinylation of Biomolecules: Attach biotin to peptides, oligonucleotides, proteins, or small molecules.
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Orthogonal Bioconjugation: Perform stepwise labeling with selective DMTr deprotection.
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Assay and Diagnostic Development: Prepare biotin-labeled probes for ELISA, western blotting, and biosensors.
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Affinity Purification: Create biotinylated ligands for streptavidin-based pull-down assays.
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Surface Functionalization: Immobilize biomolecules on streptavidin-coated microplates, beads, or sensors.
Storage and Handling:
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Storage: Store at -20°C, in a dry, dark environment.
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Handling: Use anhydrous solvents such as DMSO or DMF. Avoid exposure to moisture to preserve DMTr-protected functionality.
This product offers a balanced design of biotin affinity, PEG2 flexibility, and orthogonal reactivity, making it an especially excellent tool for controlled bioconjugation and assay development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
