DiI (DiIC18(3)): Comprehensive Guide and Applications
Overview
DiI, lipophilic tracer (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine) is an orange-red fluorescent carbocyanine dye used to label cell membranes. It incorporates its long hydrocarbon chains into lipid bilayers, becoming fluorescent upon integration. DiI is highly effective for staining entire cells, neuron tracing, and tracking cell migration.
Key Features
- Membrane Staining: DiI inserts into lipid bilayers and diffuses laterally, staining the whole cell.
- Anterograde and Retrograde Tracing: Used to trace neurons in both directions.
- Stable Labeling: No transfer between intact cells; transfer may occur with membrane disruption.
Applications
- Neurobiology: Visualizes neuronal pathways.
- Cell Biology: Tracks cell morphology and migration.
- Dual-Color Imaging: Often used with other tracers like DIA and DiO for multi-color studies.
Benefits
- Bright, Stable Fluorescence: Provides clear imaging of cell membranes.
- Versatile: Suitable for live and fixed cells in diverse studies.
Conclusion
DiI’s strong lipophilicity and bright, long-lasting fluorescence make it a valuable tool for studying cellular and neuronal structures in both research and clinical applications. Its versatility in dual-color imaging further enhances its utility in complex biological studies.
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