DBCO-SS-PEG4-Biotin is a cleavable, copper-free biotinylation reagent designed for SPAAC reactions with azide groups. Key features include:
- DBCO Moiety: Enables copper-free click chemistry.
- Cleavable Disulfide Bond: Allows reversible biotin release under reducing conditions with DTT or GSH.
- PEG4 Spacer: Enhances solubility and reduces steric hindrance.
Applications:
- Affinity Purification: Reversible biotinylation for protein capture and release.
- Pull-Down Assays: Captures protein complexes via biotin-streptavidin affinity.
- Live-Cell Labeling: Safe labeling of cell surface proteins in live cells.
- Proteomics Studies: Facilitates study of protein interactions with a cleavable tag.
Recommended storage is -20°C, with solvents such as DCM, THF, acetonitrile, DMF, or DMSO for dissolution.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
