DBCO-C6-Sulfo-NHS ester
This product is a water-soluble heterobifunctional linker featuring a dibenzocyclooctyne (DBCO) group, a six-carbon (C6) spacer, and a sulfo-NHS ester. The DBCO enables rapid, copper-free SPAAC click chemistry with azides, while the sulfo-NHS ester reacts efficiently with primary amines to form stable amide bonds. The sulfonated structure improves aqueous solubility, making it especially useful for direct bioconjugation of proteins, antibodies, and biomolecules.
Key Features and Benefits
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DBCO Group: Enables fast, copper-free strain-promoted azide–alkyne cycloaddition (SPAAC).
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Sulfo-NHS Ester: Couples selectively with amines in aqueous systems.
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C6 Spacer: Provides flexibility and spacing for efficient conjugation.
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Water-Soluble: Optimized for bioconjugation in physiological buffers.
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High Purity: Produced to ≥95% purity for reproducible results.
Applications
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Protein and Antibody Labeling: Attaches DBCO tags directly to lysine residues.
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Bioconjugation: Links azide-modified probes or carriers to amine-functionalized biomolecules.
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Probe Development: Builds dual-reactive probes for imaging and diagnostics.
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Drug Delivery: Supports copper-free click chemistry for targeted conjugates.
Storage and Handling
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Storage: Store at –20 °C in a sealed, moisture- and light-protected container.
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Handling: Dissolve freshly in aqueous buffer; avoid repeated freeze–thaw cycles.
This product combines DBCO click chemistry with sulfo-NHS amine reactivity in a water-soluble format, making it especially ideal for efficient bioconjugation and probe development.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
