Cyanine3B is recognized as one of the most luminescent fluorophores, characterized by its exceptional sensitivity. The Cyanine3B dye maleimide is typically employed to establish maleimide-thiol conjugates by attaching thiols to antibodies, peptides, and a variety of other biomolecules. This results in conjugates that are enriched with the Cyanine3B fluorophore, known for its impressive fluorescence quantum yield and photostability.
The technique leverages the Michael-addition of a thiol to a maleimide, providing a distinctive method for antibody labeling. It’s essential to note that Cyanine3B is a non-sulfonated cyanine dye, necessitating the use of an organic co-solvent like DMF or DMSO to achieve effective labeling in water buffers.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
