CalFluor 488 Azide: Advanced Fluorogenic Dye for No-Wash Bioorthogonal Labeling
Overview
CalFluor 488 Azide, CAS 1798305-98-2, is a fluorogenic dye designed for precise labeling in bioorthogonal chemistry. It reacts specifically with alkyne groups and is non-fluorescent until activated, making it ideal for no-wash imaging in live cells, tissues, and in vivo.
Key Features
- Fluorogenic Activation: Minimizes background noise by only becoming fluorescent upon reaction with alkynes.
- Bioorthogonal: Reacts selectively with target molecules, reducing non-specific binding.
- No-Wash Labeling: Perfect for sensitive applications like live-cell imaging and in vivo studies.
- Versatile Use: Functions in both copper-catalyzed and metal-free click reactions.
- Broad Emission Range: Green to far-red wavelengths suitable for diverse imaging needs.
Overcoming Limitations
Traditional azide probes require washing to remove unreacted dyes, leading to background signals. CalFluor probes, developed by Carolyn Bertozzi’s group, overcome this by activating only when they react with alkynes, allowing clear imaging without wash steps.
Applications
- Live Cell Imaging: Label proteins, glycans, DNA, and RNA in no-wash conditions.
- Bioconjugation: Precise tagging of biomolecules for clear visualization.
- Microscopy: Enhances imaging without the need for washing steps.
- In Vivo: Sensitive detection in complex tissues like mouse brain slices.
- Drug Discovery: Accurate visualization with minimal background interference.
Benefits
- Clear Imaging: Only emits fluorescence upon reaction, reducing noise.
- No Wash Steps: Saves time and preserves sample integrity.
- High Specificity: Low non-specific binding, enhancing accuracy.
Quick Tips
- Start with Recommended Doses: Adjust as needed for best results.
- Minimize Bleaching: Use anti-fade agents during imaging.
- Verify Reactivity: Ensure your target has alkyne groups for click reactions.
Get Started
Elevate your imaging with CalFluor 488 Azide. Contact us for details or to order today!
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