Biotin Azide Plus
Biotin Azide Plus is a powerful biotinylation reagent for copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. Its azide group reacts quickly with alkyne, DBCO, or BCN to form a stable triazole linkage through Click Chemistry. The copper-chelating system boosts reaction speed and efficiency, making it ideal for labeling biomolecules.
The hydrophilic PEG spacer improves solubility in water and reduces steric hindrance. It also enhances flexibility, ensuring strong binding to streptavidin and avidin molecules. This versatility makes it effective for detecting low-abundance targets and achieving high sensitivity.
Key Features
- Fast and Efficient: Reacts with alkyne, DBCO, or BCN to form stable triazole linkages.
- Improved Solubility: The PEG spacer increases water solubility and reduces steric interference.
- Enhanced Binding: Provides flexibility for better binding with streptavidin and avidin.
- High Sensitivity: Works well for detecting low-abundance biomolecules.
Applications
- Bioconjugation: Labels biomolecules like proteins or nucleic acids for detection or purification.
- Proteomics: Identifies specific proteins in complex samples.
- Molecular Imaging: Visualizes biomolecules using biotin-streptavidin interactions.
Biotin Azide Plus simplifies labeling and enhances precision in a variety of research applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
