Biotin-11-CTP (Biotin-11-cytidine-5′-triphosphate) is a modified nucleotide widely used in molecular biology and biochemistry. It features a cytidine triphosphate (CTP) molecule attached to a biotin moiety via an 11-atom spacer arm. This design allows biotin labeling for detection, purification, or immobilization of nucleic acids through strong biotin-streptavidin or avidin interactions.
Key Features:
- Biotinylation: The biotin attached to CTP efficiently labels RNA or DNA during transcription, making it detectable through biotin-streptavidin binding.
- Spacer Arm: The 11-atom linker keeps the biotin away from the nucleotide to avoid hindrance during transcription, hybridization, or binding assays.
Biotin-11-CTP Applications:
- Incorporates into RNA or DNA during in vitro transcription.
- Supports nucleic acid hybridization assays.
- Purifies biotinylated RNA or DNA with streptavidin-coated magnetic beads.
- Amplifies signals in detection assays like ELISA and Western blotting.
Typical Usage:
- In Vitro Transcription: Label RNA for detection in Northern blotting or in situ hybridization.
- Nucleic Acid Pull-down Assays: Biotinylated nucleotides help capture RNA/DNA complexes with streptavidin-coated beads, making it easier to purify nucleic acids or interacting proteins.
This product is ideal for labeling nucleic acids in a wide range of experimental workflows.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
