Tyramide signal amplification (TSA) in green fluorescent reagents is widely used in IHC, ICC, FISH, and multicolor FISH. Tyramide Signal Amplification (TSA) has increased detection sensitivity and has proven to be a particularly versatile and powerful enzymatic amplification technique. TSA is based on the ability of HRP in the presence of low concentrations of hydrogen peroxide to convert labeled tyramine-containing substrates to oxidized, highly reactive radicals that can covalently bind to tyrosine residues at or near the HRP .
APDye 488 Tyramide is part of many tyramide signal amplification kits, including SuperBoost®. This reagent can be used with any HRP-conjugated antibody for tyramide signal amplification for detection of low levels in multiplexed fluorescence immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH). Abundance targets.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
