AMCA NHS Ester Overview
AMCA NHS Ester (succinimidyl-7-amino-4-methylcoumarin-3-acetic acid, CAS 113721-87-2) is a blue fluorescent dye commonly used for labeling antibodies, proteins, and small molecules. It’s ideal for applications like immunofluorescence microscopy, arrays, and in situ hybridization due to its bright fluorescence, large Stokes shift, and resistance to photobleaching.
Key Features
- Bright Blue Fluorescence: High-contrast blue emission, perfect for imaging.
- Large Stokes Shift: Minimizes background, enhancing signal clarity.
- Photobleaching Resistance: Retains fluorescence in prolonged imaging.
- Specific Reactivity: Efficiently binds primary amines (e.g., lysine) in proteins.
Applications
- Immunofluorescence Microscopy: Used for double and triple labeling with minimal overlap.
- Arrays & Hybridization: Offers reliable, stable fluorescence for molecular analysis.
- Bioconjugation: Conjugates to proteins, peptides, and oligonucleotides, providing robust labeling.
Usage
- Reacts at pH 7-9, forming stable covalent bonds with primary amines. Store in a cool, dry place, protected from light.
Why Use AMCA NHS Ester?
- Provides clear, long-lasting fluorescence with minimal background noise. Suitable for demanding imaging and labeling tasks.
AMCA NHS Ester is your go-to for high-contrast, durable blue fluorescent labeling in bioimaging and diagnostic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
