AMCA Acid (7-Amino-4-methylcoumarin-3-acetic acid)
AMCA Acid, or 7-amino-4-methylcoumarin-3-acetic acid, is a go-to blue fluorescent dye in biochemical research. Its strong fluorescence, large Stokes shift, and resistance to photobleaching make it perfect for high-contrast imaging and precise labeling in various applications.
Key Highlights
- Fluorescent Powerhouse: Ideal for double and triple labeling in immunofluorescence microscopy.
- High Stability: Resists photobleaching, making it reliable for long-term imaging.
- Bright and Clear: Its large Stokes shift reduces background noise, enhancing visibility.
Spectral Properties
- Excitation Max: 348 nm
- Emission Max: 435 nm
- Molar Extinction Coefficient (ε): 17,400 L⋅mol⁻¹⋅cm⁻¹
- Fluorescence Quantum Yield: 0.91
- Correction Factors: CF260 = 0.16, CF280 = 0.13
These properties ensure bright, distinct blue fluorescence with minimal interference.
Applications
- Immunofluorescence Microscopy: Use AMCA Acid for high-contrast, multi-labeling studies.
- Custom Activated Esters: Prepare specialized esters like sulfo-NHS, TFP, and STP, which aren’t commercially available.
- Peptide Modification: Modify peptides during solid-phase synthesis using in-situ activation with reagents like HATU.
- Controls and Calibration: Reliable for control experiments and precise calibration.
Why Use AMCA Acid?
AMCA Acid delivers high brightness, stability, and versatility across numerous applications. Its robust fluorescence and minimal photobleaching make it an essential tool for researchers who need clear, consistent results.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
