AF 594 Tyramide is a fluorescent dye commonly used in tyramide signal amplification (TSA) techniques for immunohistochemistry (IHC), immunocytochemistry (ICC), and in situ hybridization (ISH). It provides high sensitivity due to its ability to covalently bind to proteins in tissue samples after enzymatic activation.
Key Features:
- Fluorophore: Alexa Fluor 594 (AF 594)
- Excitation/Emission: 590 nm / 617 nm (red fluorescence)
- Application: Signal amplification in enzyme-linked assays (e.g., HRP-conjugated antibodies).
How It Works:
- Enzyme Activation: Horseradish peroxidase (HRP) catalyzes AF 594 tyramide in the presence of hydrogen peroxide (H2O2).
- Covalent Binding: The activated tyramide forms covalent bonds with nearby tyrosine residues on proteins.
- Signal Visualization: The covalent labeling results in enhanced and localized fluorescence.
Applications:
- IHC/ICC: For detecting low-abundance proteins.
- ISH: For nucleic acid detection with enhanced sensitivity.
- Multiplexing: Due to its red fluorescence, AF 594 tyramide is suitable for multiplex imaging alongside other fluorophores.
Storage & Handling:
- Storage Temperature: -20°C (protected from light).
- Working Concentration: Typically 1:100 to 1:500 dilution in a TSA working buffer.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
