3′-O-DMTr-thymidine 5′-CE Phosphoramidite | CAS 134031-86-0
Overview:
3′-O-DMTr-thymidine 5′-CE Phosphoramidite (CAS: 134031-86-0) is a key reagent used in DNA oligonucleotide synthesis. This product enables the stepwise addition of thymidine bases during solid-phase synthesis, making it essential for the creation of custom DNA sequences for research, diagnostics, and therapeutic applications.
Key Features:
- CAS Number: 134031-86-0
- Molecular Formula: C43H52N3O7P
- Molecular Weight: 752.88 g/mol
- Synonyms: Thymidine phosphoramidite, 3′-O-(4,4′-dimethoxytrityl)-thymidine 5′-O-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
Applications:
- DNA Oligonucleotide Synthesis: Primarily used in automated DNA synthesizers for creating custom oligonucleotide sequences. It plays a crucial role in research labs and industries producing DNA primers, probes, and therapeutic oligonucleotides.
- Therapeutic Oligonucleotides: The synthetic DNA produced with 3′-O-DMTr-thymidine 5′-CE phosphoramidite is used in antisense therapies, siRNA, and gene editing technologies.
- PCR Primers and Probes: This compound helps in generating short, custom DNA sequences that are critical for PCR and real-time PCR assays in research and diagnostic settings.
- Genetic Engineering: Enables precision in gene synthesis projects for genomic studies, cloning, and sequencing applications.
Product Benefits:
- High Purity: Ensures the accuracy and efficiency of oligonucleotide synthesis.
- Protective Groups: The DMTr (dimethoxytrityl) group protects the 3′-hydroxyl group during DNA chain assembly, and the CE (cyanoethyl) group facilitates coupling, ensuring high yield and fidelity.
- Custom DNA Sequences: Allows for the stepwise, controlled addition of thymidine in custom DNA strands.
Usage Guidelines:
- Storage: Store the compound under dry, inert conditions, such as in an argon- or nitrogen-purged environment, to avoid moisture degradation.
- Handling: Use appropriate lab protocols when working with this phosphoramidite to ensure stability and efficacy during synthesis.
Why Choose 3′-O-DMTr-thymidine 5′-CE Phosphoramidite?
- Trusted Quality: Used by leading research institutions and biotech companies for reliable DNA synthesis.
- Scalable for Large Projects: Suitable for both small and large-scale oligonucleotide production, from basic research to clinical applications.
- Optimized for Automation: Compatible with automated DNA synthesizers, making it a cost-effective and time-saving solution for high-throughput DNA production.
Specifications:
- Appearance: White to off-white powder
- Purity: ≥98%
- Storage: -20°C in a dry environment
- Solubility: Soluble in acetonitrile or similar organic solvents
FAQs:
What is 3′-O-DMTr-thymidine 5′-CE Phosphoramidite used for?
It’s used in solid-phase DNA synthesis, enabling the sequential addition of thymidine bases in custom oligonucleotides for research, diagnostics, and therapeutic applications.
How should I store 3′-O-DMTr-thymidine 5′-CE Phosphoramidite?
Store it at -20°C in a moisture-free environment to prevent degradation.
What types of projects can benefit from this product?
Ideal for PCR primers, antisense oligonucleotides, gene sequencing, and molecular biology research.
Order Now:
Contact us today for pricing information or to place an order for 3′-O-DMTr-thymidine 5′-CE Phosphoramidite (CAS: 134031-86-0). Take your oligonucleotide synthesis to the next level with a trusted reagent that delivers high-quality results.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
