DSPE-Polysarcosine-Mal20

Key Properties

• A trifunctional amphiphilic lipid conjugate composed of a hydrophobic DSPE anchor, a hydrophilic polysarcosine (pSar20) spacer, and a reactive maleimide end group.
• The polysarcosine segment provides excellent antifouling (stealth) performance, high hydrophilicity, good biocompatibility, and low immunogenicity, acting as a biodegradable alternative to PEG.
• The maleimide group reacts specifically and efficiently with thiol groups (-SH) on cysteine-containing peptides, proteins, and ligands under mild neutral conditions to form stable thioether bonds.
• Can stably insert into lipid bilayers while exposing reactive maleimide moieties on the nanoparticle surface.
• Soluble in organic solvents including chloroform, methanol, and DMSO; dispersible in aqueous buffers.
• Usually supplied as a white to off-white solid or waxy powder.
• Storage: −20 °C, desiccated, protected from light and oxidation to prevent maleimide hydrolysis.

Applications

• Used as a reactive stealth lipid for surface functionalization of liposomes, lipid nanoparticles (LNPs), micelles, and exosomes.
• Enables covalent conjugation with thiol-modified targeting ligands, antibodies, peptides, aptamers, and fluorescent probes.
• Reduces nonspecific protein adsorption and prolongs blood circulation time of nanocarriers in drug and nucleic acid (mRNA, siRNA, DNA) delivery.
• Applied in targeted drug delivery, vaccine formulation, and in vitro/in vivo molecular imaging.
• Serves as a PEG alternative to avoid accelerated blood clearance (ABC) and immune responses related to PEG lipids.
• Used for biomembrane engineering and surface modification of biomaterials with improved biocompatibility.