Hoechst 34580 (bisbenzimide, HOE 34580), CAS Number: 911004-45-0
Hoechst 34580 is a blue fluorescent dye that binds selectively to A-T-rich regions of DNA. It is widely used in microscopy and flow cytometry for DNA visualization and cell analysis.
Key Features:
- Fluorescence:
- Excitation ~380 nm, Emission ~438 nm (blue fluorescence).
- Unbound dye fluoresces green (510–540 nm) at high concentrations.
- DNA Binding: Strong preference for A-T-rich regions.
- Cell Permeability: Effective for live and fixed cells
- Low Toxicity: Less toxic than DAPI
- BrdU Sensitivity: Quenched by bromodeoxyuridine (BrdU), useful for cell cycle studies.
- Stokes Shift: Suitable for multicolor labeling with minimal overlap.
Hoechst 34580 Applications:
- DNA Staining: Chromosome and nuclear visualization in microscopy.
- Flow Cytometry: Cell cycle analysis and DNA quantification.
- Apoptosis Detection: Identifying condensed nuclei in apoptotic cells.
- Cell Sorting: Nuclear staining for cell sorting.
- Multicolor Imaging: Works well in multiplexed experiments.
- Cell Cycle Studies: Leveraging BrdU quenching for tracking cell division.
Storage and Handling:
- Store at -20°C, protected from light.
- Soluble in water, DMSO, and standard buffers.
Hoechst 34580 is an essential dye for DNA-specific staining in various biological applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
