Bis-sulfone-PEG12-NHS Ester (CAS: 2055105-51-4)
Bis-sulfone-PEG12-NHS Ester is ideal for precise bioconjugation and protein crosslinking. It selectively targets two cysteine thiol groups from a disulfide bond, forming a stable thioether bridge while maintaining the protein’s structure. This ensures efficient PEGylation without denaturation.
Mechanism of Action:
First, the disulfide bond is reduced to release the cysteine thiols. Then, the bis-sulfone group reacts with both thiols through bis-alkylation, forming a thioether bridge. Simultaneously, the PEG12 spacer attaches, adding flexibility and solubility.
Key Features:
- Thiol-Selective: Specifically targets cysteine thiols for precise conjugation.
- Bis-alkylation: Forms a strong thioether bridge while preserving protein structure.
- PEG12 Spacer: Improves solubility and ensures single PEG attachment.
- NHS Ester: Reacts quickly with amines, accelerating bioconjugation.
Applications:
- Protein PEGylation: Perfect for PEGylating proteins, peptides, and enzymes.
- Crosslinking: Forms stable bridges between cysteines, boosting stability.
- Biopharmaceuticals: Enhances drug stability without altering function.
Advantages:
- Structure Preservation: Keeps the protein’s natural folding and activity intact.
- Thiol Selectivity: Prevents over-conjugation, ensuring precise modification.
- Efficient PEGylation: Guarantees one PEG per disulfide bond, reducing side reactions.
- Cost-Effective: Allows for recycling of unreacted proteins, lowering costs.
This reagent is perfect for researchers needing efficient, cost-effective protein modification in biopharmaceuticals and PEGylation projects.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
