Bis-sulfone Amine (C3), also known as Bis-sulfone Amine, is a versatile bis-alkylating labeling reagent. It selectively targets cysteine sulfur atoms in native disulfide bonds, allowing for precise bioconjugation. These reagents bis-alkylate to link both thiols from two cysteine residues in a reduced disulfide bond, like those in antibody interchain disulfide bonds. This reaction creates a covalent bridge using a three-carbon linker, preserving the protein’s structure.
Moreover, Bis-sulfone Amine (C3) can attach PEG to a polyhistidine tag (His-tag) on proteins, ensuring site-specific conjugation. It also reacts efficiently with activated carboxylic acid groups on biomolecules, making bioconjugation both targeted and effective.
Key Features:
- Function: Bis-alkylating reagent for accurate cysteine targeting
- Selectivity: Rebridges native disulfide bonds for stability
- Versatility: Links PEG to His-tagged proteins and interacts with activated carboxylic acids
- Applications:
- Antibody-drug conjugates (ADCs)
- Protein and peptide labeling
- Targeted drug delivery
- Bioconjugation and cross-linking
Ref:
Khalili, H., et al. (2012). Comparative Binding of Disulfide-Bridged PEG-Fabs. Bioconjug. Chem., 23(11), 2262-77.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.