YO-TAP-3 (Oxazole Red Monomer, YO-PRO®-3, CAS 157199-62-7)
YO-TAP-3, also known as Oxazole Red Monomer or YO-PRO®-3, is a far-red fluorescent dye commonly used for nucleic acid staining. Specifically, it binds strongly to double-stranded DNA (dsDNA) and RNA (dsRNA). Upon binding, it becomes highly fluorescent, which makes it particularly well-suited for applications such as cell viability assays and apoptosis detection.
Key Features:
- Fluorescence Properties:
- Excitation Maximum: ~612 nm
- Emission Maximum: ~631 nm
- Produces bright far-red fluorescence upon nucleic acid binding.
- Selective Binding:
- Binds specifically to dsDNA and dsRNA with minimal background fluorescence.
- Cell Impermeant:
- Stains only dead or apoptotic cells with compromised membranes.
- Far-Red Spectrum:
- Suitable for multicolor experiments with minimal overlap with other fluorophores.
Applications:
- Nucleic Acid Detection:
- Stains dsDNA and dsRNA for use in microscopy and flow cytometry.
- Cell Viability and Apoptosis Assays:
- Works well in combination with cell-permeable dyes like LUCS 13 or Annexin V-AF488 to simultaneously label live, apoptotic, and dead cells.
- Apoptosis Detection:
- Highlights early apoptotic cells due to membrane changes.
- Chromosome and Nuclear Staining:
- Provides clear nuclear visualization in multicolor fluorescence imaging.
- Microarrays:
- Enables high-sensitivity nucleic acid detection in high-throughput studies.
Handling and Storage:
- Form: Supplied as a DMSO or aqueous buffer solution.
- Storage: Store at -20°C, protected from light.
- Precautions: Use protective equipment and handle in a well-ventilated area.
YO-TAP-3 is a valuable tool for nucleic acid staining and apoptosis studies, offering bright fluorescence and high specificity. Its compatibility with cell-permeable dyes like LUCS 13 and Annexin V-AF488 enhances its utility in assessing cell viability and apoptosis simultaneously.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
