TCO-PEG4-DBCO
This product is a heterobifunctional linker with a trans-cyclooctene (TCO) group, a PEG4 spacer, and a dibenzocyclooctyne (DBCO) group. The TCO reacts rapidly with tetrazines via copper-free IEDDA ligation, while the DBCO undergoes SPAAC click chemistry with azides. The PEG4 spacer provides solubility, flexibility, and moderate spacing, making this reagent especially effective for dual-functional bioconjugation.
Key Features and Benefits
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TCO Group: Enables ultrafast, copper-free IEDDA ligation with tetrazines.
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DBCO Group: Performs strain-promoted click chemistry with azides.
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PEG4 Spacer: Improves solubility, accessibility, and reduces steric hindrance.
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Dual Reactivity: Combines two orthogonal click reactions in one molecule.
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High Purity: Manufactured to ≥95% purity for consistent performance.
Applications
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Bioconjugation: Links tetrazine- and azide-modified biomolecules.
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Protein and Antibody Labeling: Provides orthogonal handles for multifunctional modifications.
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Probe Development: Builds dual-reactive probes for imaging and diagnostics.
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Drug Delivery: Functions as a modular linker in therapeutic design.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Use freshly prepared solutions; avoid repeated freeze–thaw cycles.
This product combines ultrafast IEDDA reactivity with strain-promoted click chemistry in a PEG4 scaffold, specifically offering versatility for advanced conjugation strategies.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
