TCO-PEG1-NHS ester
This product is a high-purity crosslinker with a trans-cyclooctene (TCO) for rapid, catalyst-free IEDDA cycloaddition with tetrazines and an NHS ester for efficient amine coupling. The short PEG1 spacer specifically enhances solubility, reduces steric hindrance, and maintains compact length, enabling high-efficiency, site-specific bioconjugation in research, diagnostics, and drug delivery.
Key Features and Benefits
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TCO Group – Enables ultra-fast, catalyst-free IEDDA click chemistry with tetrazines for bioorthogonal conjugation.
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NHS Ester – Reacts efficiently with primary amines on proteins, peptides, and other biomolecules.
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PEG1 Spacer – Improves solubility, reduces steric hindrance, and maintains a compact linker length.
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Orthogonal Design – Allows sequential or dual labeling strategies without cross-reactivity.
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High Purity – Supplied at >95% purity for reproducible results in sensitive applications.
Applications
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Bioorthogonal labeling of proteins, peptides, and nucleic acids
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Antibody–drug conjugate (ADC) linker chemistry
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Site-specific drug delivery systems
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Multifunctional imaging and diagnostic probe development
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Surface modification of biomaterials and nanoparticles
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged exposure to moisture to preserve NHS ester reactivity
This product integrates ultra-fast tetrazine ligation via TCO with efficient amine coupling through NHS ester chemistry, enhanced by a short PEG1 spacer for solubility and minimal steric hindrance, making it especially ideal for precise and orthogonal bioconjugation.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
