Tetramethylrhodamine (TAMRA) is a xanthene dye with orange emission. The dye is a FRET acceptor for FAM, and sometimes used as a quencher for it.
Like other xanthenes, TAMRA exists as two isomers (5- and 6-), which have very similar spectral properties. This is an azide derivative of 6-isomer of TAMRA. The azide can be conjugated with terminal alkynes using copper-catalyzed Click chemistry, or with cycloalkynes with copper-free strain promoted alkyne azide cycloaddition (spAAc) reaction.
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