Sulfo-Cyanine5 Maleimide (CAS: 2242791-82-6) is a water-soluble fluorescent dye commonly used in bioconjugation. It contains a maleimide group that reacts specifically with thiol groups, enabling the labeling of proteins, peptides, and other thiol-containing molecules. Its far-red fluorescence properties make it suitable for various biological applications.
Key Features
- Spectral Properties: Excitation at 646 nm and emission at 662 nm for far-red fluorescence.
- High Solubility: Sulfonate groups improve water solubility for biological use.
- Specific Reactivity: Maleimide group selectively targets thiol groups, ideal for cysteine residue labeling.
Applications
- Protein Labeling: Used to attach fluorescent tags to proteins for tracking and visualization.
- Fluorescence Microscopy: Provides bright and stable signals for imaging.
- Flow Cytometry: Serves as a marker for cell analysis and sorting.
Specifications
- Molecular Formula: C₃₈H₄₃KN₄O₉S₂
- Molecular Weight: 803.00 g/mol
- Storage: Store at -20°C in the dark to maintain stability.
An analog without sulfo groups, Cyanine5 maleimide, is also available.
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