NThiol-SS-biotin

NThiol-SS-biotin

NThiol-SS-biotin is a high-purity biotin linker with a terminal thiol for selective coupling and a cleavable disulfide (SS) bond specifically for controlled release. The design enables reversible and site-specific conjugation in diverse systems.

Key Features and Benefits

• Thiol Group – Reacts selectively with maleimides, haloacetyls, and gold surfaces

• Disulfide Linkage – Cleavable under mild reducing conditions for reversible release

• Biotin Moiety – Strong, specific binding to streptavidin and avidin proteins

• Orthogonal Reactivity – Supports sequential modifications without interference

• High Purity – >95% for reproducible bioconjugation performance

Applications

• Reversible biotinylation of biomolecules

• Affinity purification and pull-down assays

• Antibody and protein labeling

• Targeted drug delivery with controlled release

• Biosensor and diagnostic development

Storage and Handling

• Storage: Store at –20 °C, dry, and protected from light
• Handling: Use anhydrous solvents; protect thiol from oxidation by working under inert conditions or adding stabilizers

NThiol-SS-biotin combines a reactive thiol, cleavable disulfide, and biotin specifically for reversible, high-affinity conjugation in bioconjugation, purification, and delivery applications.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.