Mal-PEG3-CH2 tBu-ester
Mal-PEG3-CH2 tBu-ester is a protected heterobifunctional PEG linker containing a maleimide group, a PEG3 spacer, and a terminal t-butyl ester. The maleimide specifically enables selective conjugation with thiols to form stable thioether bonds. The PEG3 spacer improves solubility and reduces steric hindrance, while the t-butyl ester protects the carboxylic acid for controlled deprotection and downstream functionalization.
Key Features and Benefits
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Maleimide Group: Reacts selectively with thiols (e.g., cysteine residues) to form stable thioether bonds.
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PEG3 Spacer: Enhances solubility and flexibility, improving conjugation efficiency.
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t-Butyl Ester Protection: Masks the carboxyl group, removable under mild acidic conditions for further coupling.
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Orthogonal Reactivity: Supports stepwise conjugation strategies with precise control.
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High Purity: Produced to ≥95% purity for reproducible results.
Applications
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Bioconjugation: Couples thiol-containing biomolecules while offering a protected acid for later derivatization.
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Drug Delivery: Serves as an intermediate for linker design in ADCs and nanocarriers.
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Probe Development: Enables multifunctional probe synthesis through thiol conjugation and acid deprotection.
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Organic Synthesis: Useful as a modular PEG linker for controlled dual functionalization.
Storage and Handling
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Storage: Store at –20 °C in a sealed, light- and moisture-protected container.
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Handling: Dissolve freshly in dry solvent before use; deprotect t-butyl ester under mild acidic conditions as required.
Mal-PEG3-CH2 tBu-ester combines thiol-reactive maleimide chemistry with a protected acid group, specifically providing a versatile intermediate for staged conjugation, probe development, and drug delivery research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
