m-PEG8-amido-Mal
m-PEG8-amido-Mal is a monofunctional PEG linker featuring a maleimide (Mal) group for thiol-specific conjugation and a methoxy-capped PEG8 spacer for enhanced solubility and moreover flexibility. Designed for site-specific bioconjugation, this reagent enables stable thioether bond formation with cysteine residues in proteins, peptides, and other thiol-containing molecules.
Key Features and Benefits:
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Maleimide Functional Group: Selectively reacts with free thiols at physiological pH to form stable thioether bonds.
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PEG8 Spacer: Provides improved aqueous solubility, extended reach, and reduced steric hindrance.
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Methoxy-Capped Terminus: Prevents further reactivity, making the compound ideal for monofunctional PEGylation.
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Amide Bond Backbone: Enhances hydrolytic stability and minimizes undesired side reactions.
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High Purity: Supplied at >95% purity, ensuring reliable performance in sensitive bioconjugation workflows.
Applications:
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Thiol-Specific Bioconjugation: Ideal for labeling cysteine-containing proteins, antibodies, and peptides.
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Surface PEGylation: Used to passivate nanoparticles or medical devices, reducing nonspecific binding.
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Protein Modification: Increases solubility and circulation half-life of therapeutic proteins.
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Site-Specific PEGylation: Facilitates precise, controlled attachment of PEG to biomolecules.
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Nanomedicine and Drug Delivery: Improves pharmacokinetics and biocompatibility of drug carriers.
Storage and Handling:
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Storage: Store at -20°C, protected from light and moisture.
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Handling: Reconstitute in dry, oxygen-free solvents (e.g., DMSO or DMF). Use freshly prepared solutions to avoid maleimide hydrolysis.
m-PEG8-amido-Mal offers a reliable solution for thiol-targeted conjugation with excellent solubility, minimal immunogenicity, and consistent performance, consequently making it a preferred choice for PEGylation, surface passivation, and advanced bioconjugation applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
