FmocNH-PEG2-CH2CO2-NHS ester
FmocNH-PEG2-CH2CO2-NHS ester is a high-purity heterobifunctional PEG linker with an Fmoc-protected amine for orthogonal synthesis and an NHS ester-activated carboxylic acid for rapid amine coupling. The PEG2 spacer enhances solubility, flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. The Fmoc group protects the amine until selective deprotection is desired, allowing stepwise conjugation strategies.
Key Features and Benefits
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Fmoc-Protected Amine: Stable under mild conditions; removable with piperidine to reveal a reactive primary amine.
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NHS Ester: Couples rapidly with primary amines under mild conditions to form stable amide bonds.
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PEG2 Spacer: Improves solubility, flexibility, and accessibility of reactive groups.
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Heterobifunctional Design: Supports orthogonal and sequential modification strategies.
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High Purity: Supplied at >95% purity for consistent and reproducible results.
Applications
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Stepwise Bioconjugation: Protect the amine until a later stage for controlled functionalization.
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Peptide and Protein Modification: Couple the NHS ester first, then deprotect the amine for further coupling.
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Nanoparticle and Surface Functionalization: Add PEG spacers with latent amine functionality.
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Drug Delivery Systems: Enable multi-step linker–payload assembly with controlled activation points.
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Diagnostic and Imaging Probes: Create multifunctional probes with orthogonal reactive groups.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid prolonged moisture exposure to preserve NHS ester reactivity.
FmocNH-PEG2-CH2CO2-NHS ester combines an orthogonally protected amine with an activated ester in a hydrophilic PEG2 framework, offering versatility for multi-step bioconjugation in therapeutics, diagnostics, and materials science.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
