Fluorescein Isothiocyanate (FITC): Key Information
Fluorescein Isothiocyanate (FITC) is a popular fluorescent dye that emits a yellow-green color. It has an absorption maximum at 495 nm and an emission maximum at 525 nm.
Key Features
- Fluorescence: FITC emits bright yellow-green fluorescence, making it ideal for sensitive detection and imaging.
- Reactive Group: The isothiocyanate group reacts with nucleophiles like amino or thiol groups, allowing it to label proteins and other biomolecules.
Applications
- Protein Labeling: Commonly used to tag proteins, including antibodies and lectins, for visualization in microscopy and flow cytometry.
- Immunoassays: Enhances the detection of specific antigens or antibodies through fluorescent labeling.
- Cell Biology: Facilitates the study of cellular structures and processes by labeling cellular components.
Advantages
- High Sensitivity: Offers strong and clear fluorescence for precise detection.
- Versatility: Effective for labeling a variety of biomolecules due to its reactive isothiocyanate group.
Fluorescein Isothiocyanate (FITC) is a reagent-grade dye used for advanced research and fluorescence applications. For GMP-grade options, please contact us.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
