endo-BCN-PEG3-NHBoc
endo-BCN-PEG3-NHBoc is a high-purity heterobifunctional PEG linker with an endo-bicyclo[6.1.0]nonyne (BCN) group for rapid, catalyst-free strain-promoted azide–alkyne cycloaddition (SPAAC) and a t-Boc–protected amine for orthogonal conjugation. The PEG3 spacer improves aqueous solubility, enhances flexibility, and reduces steric hindrance, enabling efficient bioconjugation in both aqueous and organic systems. The acid-labile t-Boc group allows controlled deprotection for stepwise functionalization, making it ideal for precision linker design in drug delivery, diagnostics, and biomaterials.
Key Features and Benefits
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Endo-BCN Functional Group: Enables rapid, copper-free click chemistry with azides under mild conditions.
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t-Boc–Protected Amine: Allows orthogonal, controlled deprotection for sequential conjugation.
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PEG3 Spacer: Improves solubility, flexibility, and accessibility of reactive sites.
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Heterobifunctional Design: Supports multi-step, orthogonal bioconjugation workflows.
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High Purity: Supplied at >95% purity for reproducible, high-yield performance.
Applications
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Bioorthogonal Labeling: Attach BCN handles for SPAAC click reactions with azide-containing biomolecules.
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Protein and Peptide Modification: Reserve amine functionality for later conjugation steps.
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Antibody–Drug Conjugates (ADCs): Enable site-specific drug attachment with orthogonal reactivity.
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Nanoparticle Functionalization: Add bioorthogonal handles for targeted delivery systems.
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Diagnostic Probes: Create multifunctional imaging and sensing agents.
Storage and Handling
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Storage: Store at –20 °C, dry, and protected from light.
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Handling: Use anhydrous solvents such as DMF or DMSO; avoid moisture to preserve BCN and Boc group integrity.
endo-BCN-PEG3-NHBoc combines BCN click reactivity with a protected amine in a hydrophilic PEG3 framework, offering orthogonal conjugation capability for multifunctional bioconjugates, drug delivery, and diagnostic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
