DMTR-biotin-PEG3-(2-cyanoethyl) diisopropylphosphoramidite
This product is a high-purity reagent for incorporating biotin into oligonucleotides via automated phosphoramidite synthesis. It features a DMTr-protected biotin, a PEG3 spacer for solubility and reduced steric hindrance, and a 2-cyanoethyl diisopropylphosphoramidite group for efficient coupling, making it especially ideal for producing biotin-labeled probes, primers, and capture oligonucleotides.
Key Features and Benefits
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DMTr-Protected Biotin: Enables controlled biotin incorporation during synthesis.
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Phosphoramidite Group: Compatible with automated DNA/RNA synthesizers for efficient coupling.
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PEG3 Spacer: Enhances solubility and reduces steric hindrance in oligonucleotide assembly.
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High Purity: Supplied at >95% purity for consistent, high-yield synthesis.
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Versatile Utility: Suitable for diverse downstream applications involving biotin–streptavidin binding.
Applications
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Synthesis of biotin-labeled oligonucleotides for detection and capture.
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Preparation of biotinylated primers and probes for PCR, qPCR, and hybridization assays.
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Oligonucleotide immobilization on streptavidin-coated surfaces.
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Affinity purification of nucleic acids.
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Biosensor and diagnostic assay development.
Storage and Handling
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Storage: Store at –20 °C under inert gas, dry, and protected from light.
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Handling: Use anhydrous acetonitrile for coupling; avoid moisture to maintain reactivity.
This product enables efficient biotin incorporation into oligonucleotides via automated phosphoramidite chemistry, with a PEG3 spacer specifically for solubility and flexibility in high-performance probe and primer synthesis.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
