DMT-5Me-dC(Bz)-CE Phosphoramidite
This product is a DNA synthesis building block designed for the incorporation of 5-methyl-2′-deoxycytidine into oligonucleotides. It specifically features a DMT (dimethoxytrityl) group protecting the 5′-hydroxyl, a benzoyl (Bz) group protecting the exocyclic amine, and a 2-cyanoethyl (CE) phosphoramidite for automated coupling. The 5-methyl substitution enhances duplex stability and mimics natural DNA methylation, making this reagent especially valuable for epigenetics, methylation studies, and custom oligonucleotide synthesis.
Key Features and Benefits
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5-Methyl Substitution: Increases duplex stability and mimics epigenetic methylation.
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Bz Protection: Ensures stability of the exocyclic amine during synthesis.
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DMT Group: Protects the 5′-hydroxyl for stepwise DNA synthesis.
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CE Phosphoramidite: Compatible with automated solid-phase oligonucleotide synthesis.
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High Purity: Manufactured to ≥98% purity for consistent synthesis performance.
Applications
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Epigenetics Research: Enables synthesis of methylated DNA for methylation studies.
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Oligonucleotide Synthesis: Incorporates 5-methyl-dC into custom DNA sequences.
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Structural Biology: Probes effects of methylation on DNA structure and stability.
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Diagnostics: Builds methylated DNA standards for assay calibration.
Storage and Handling
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Storage: Store dry at –20 °C under inert atmosphere.
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Handling: Use under anhydrous conditions; phosphoramidites are moisture-sensitive.
This product offers reliable incorporation of 5-methylcytidine into oligonucleotides, specifically supporting advanced epigenetics and nucleic acid research.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
