DMT-2′Fluoro-DG(IB) Amidite
DMT-2′Fluoro-DG(IB) Amidite is a high-purity guanosine amidite featuring a 2′-fluoro modification for especially enhanced nuclease resistance and binding affinity, an N²-isobutyryl (IB) protecting group for efficient synthesis, and a 5′-O-dimethoxytrityl (DMT) protecting group for controlled stepwise oligonucleotide assembly. The amidite functionality specifically enables rapid, efficient coupling in solid-phase synthesis, making it ideal for the preparation of chemically stabilized RNA and DNA oligonucleotides.
Key Features and Benefits
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2′-Fluoro Modification: Improves nuclease resistance, duplex thermal stability, and hybridization affinity.
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N²-Isobutyryl Protection: Minimizes side reactions during synthesis and ensures efficient deprotection.
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5′-O-DMT Group: Supports controlled, stepwise synthesis with high coupling efficiency.
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Amidite Chemistry: Compatible with standard phosphoramidite protocols for automated synthesizers.
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High Purity: Supplied at >95% purity for reproducible and consistent results.
Applications
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Therapeutic Oligonucleotides: Synthesize RNA or DNA oligos with enhanced stability for clinical applications.
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siRNA and Antisense Oligos: Improve efficacy and in vivo half-life through chemical stabilization.
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Aptamer and Ribozyme Synthesis: Create functional nucleic acids with greater durability.
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Diagnostic Probes: Develop stable probes for hybridization assays, qPCR, and imaging.
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Research Oligonucleotides: Generate chemically modified oligos for molecular biology studies.
Storage and Handling
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Storage: Store at –20 °C, dry, and under inert atmosphere.
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Handling: Use anhydrous acetonitrile or similar dry solvents; avoid moisture to maintain amidite reactivity.
DMT-2′Fluoro-DG(IB) Amidite combines 2′-fluoro stabilization, guanosine IB protection, and amidite reactivity for high-fidelity synthesis of stable, modified RNA and DNA oligonucleotides specifically in therapeutic, diagnostic, and research applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
