DMT-2′Fluoro-dU Phosphoramidite

DMT-2′Fluoro-dU Phosphoramidite

DMT-2′Fluoro-dU Phosphoramidite is a high-purity uridine derivative featuring a 2′-fluoro modification on the ribose ring and a DMT-protected 5′-hydroxyl group, with a 3′-phosphoramidite moiety for automated oligonucleotide synthesis. The 2′-fluoro substitution enhances nuclease resistance and thermal stability, making it ideal for constructing modified oligonucleotides with improved biological stability and binding affinity. Fully compatible with standard DNA/RNA synthesis protocols, it specifically enables precise incorporation into antisense, siRNA, aptamer, and structural biology studies.

Key Features

• 2′-fluoro modification for increased nuclease resistance and thermal stability

• DMT-protected 5′-OH group for automated synthesis compatibility

• High coupling efficiency with standard phosphoramidite chemistry

• Suitable for both DNA and RNA analog synthesis

Applications

• Synthesis of nuclease-resistant oligonucleotides

• Antisense and siRNA therapeutic research

• Aptamer design and optimization

• Structural studies of nucleic acid–protein interactions

Storage and Handling

• Store dry at –20 °C under inert gas

• Protect from light and moisture

• Warm to room temperature before opening

• Avoid repeated freeze–thaw cycles

This product is a high-purity 2′-fluoro uridine building block specifically for stable, nuclease-resistant oligonucleotide synthesis in DNA, RNA, and therapeutic applications.