Dde Biotin-PEG4-DBCO

Dde Biotin-PEG4-DBCO (CAS: 1807512-43-1)

Dde Biotin-PEG4-DBCO is a cleavable biotinylation reagent designed for copper-free click chemistry applications. It features a biotin group linked to a dibenzocyclooctyne (DBCO) through a PEG4 spacer. The DBCO group enables strain-promoted alkyne-azide cycloaddition (SPAAC) with azide-containing molecules, while the Dde (dimethyldioxocyclohexylidene) group allows reversible biotin release using hydrazine under mild conditions. As a result, it offers exceptional versatility for molecular biology research.

Key Features:

• Cleavable Biotin Reagent: Supports reversible biotinylation, making target capture and controlled release simple and efficient.
• Copper-Free Click Chemistry: Since the DBCO group reacts with azides without requiring a copper catalyst, it is safe for sensitive biological samples.
• PEG4 Spacer: By enhancing solubility and reducing steric hindrance, the PEG4 spacer ensures more efficient and consistent conjugation reactions.

Applications:

• Bioconjugation: Labels biomolecules for detection, purification, and immobilization, thus streamlining molecular tagging.
• Proteomics and Molecular Biology: Facilitates studies involving protein interactions, post-translational modifications, and cell-based assays, providing deeper insights into cellular functions.
• Drug Delivery Research: Assists in the creation of targeted drug delivery systems by attaching therapeutic agents to specific biomolecules, thereby advancing drug development processes.

Handling and Storage:

• Molecular Formula: C₄₇H₆₁N₅O₉S
• Molecular Weight: 872.09 g/mol
• Storage: Store at -20°C, protected from light and moisture to maintain product stability.

Dde Biotin-PEG4-DBCO offers a powerful combination of reversible biotinylation, efficient biomolecule labeling, and copper-free click chemistry compatibility. Its flexibility makes it a valuable tool for proteomics, molecular biology, and drug development research.