Cyanine5 Tetrazine (Cy5 Tetrazine) is a red-fluorescent dye combining the Cy5 fluorophore with a tetrazine functional group. It supports copper-free click chemistry by reacting quickly with trans-cyclooctene (TCO) and strained alkenes through strain-promoted inverse electron-demand Diels-Alder (SPIEDAC) reactions.
Key Features:
- Bright Red Fluorescence: Absorbs at ~646 nm and emits at ~662 nm, making it ideal for fluorescence imaging.
- Fast Reactivity: The tetrazine group reacts efficiently with TCO-containing molecules for rapid, bioorthogonal labeling.
- Water-Soluble Options: Some derivatives, such as Sulfo-Cy5-Tetrazine, dissolve easily in water, enhancing use in biological systems.
Cyanine5 Tetrazine Applications:
- Bioconjugation: Labels proteins, peptides, and nucleic acids for in vitro and in vivo experiments.
- Fluorescence Imaging: Works well for fluorescence microscopy, flow cytometry, and other imaging techniques.
- Pre-Targeted Imaging and Therapy: Supports targeted imaging and drug delivery due to its fast, selective reactions.
How to Use:
- Storage: Keep at -20°C, away from light and moisture, to maintain stability.
- Solubility: Dissolves in DMSO or DMF; water-soluble versions are available for aqueous environments.
Cyanine5 Tetrazine offers strong fluorescence, quick reactivity, and versatile bioconjugation, making it a valuable tool for molecular imaging, drug development, and diagnostic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
