Cyanine5 Phosphoramidite is one of the dyes that are used in qPCR. The corresponding filter set is found on many qPCR machines.
This reactive derivative is useful for the synthesis of qPCR probes having Cyanine5 at 5′-end, the most typical location of the fluorophore. Cyanine5 Phosphoramidite is a terminating, non-nucleoside reagent.
Amidite group in this reagent is bound to a secondary oxygen. This provides extra stability against Arbuzov rearrangement – a reaction that leads to amidite deterioration in solution in oligonucleotide synthesizer. This molecular design increases the stability of the reagent, maintaining efficient coupling over a longer time.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
