Cyanine5 NHS Ester is a widely used fluorescent dye for labeling amino groups in proteins, peptides, and oligonucleotides. As an analog of Cy5®, it provides bright red fluorescence, optimal for imaging applications due to its emission in a range where CCD detectors are highly sensitive and background interference is minimal. Even tiny amounts, as small as 1 nmol, can be seen by the naked eye in gel electrophoresis.
Spectral Properties:
- Excitation Maximum: 646 nm
- Molar Extinction Coefficient (ε): 250,000 L⋅mol⁻¹⋅cm⁻¹
- Emission Maximum: 662 nm
- Fluorescence Quantum Yield: 0.2
- CF260: 0.03
- CF280: 0.04
Key Features:
- Bright Red Fluorescence: Emits intense red light, compatible with Cy5 filter sets.
- High Sensitivity: Detects minute quantities easily, ideal for life sciences.
- Efficient Labeling: Quickly tags primary amines with minimal co-solvents like DMF or DMSO.
- Versatile Use: Replaces Cy5®, Alexa Fluor 647, and DyLight 649 in labeling protocols.
Applications:
- Protein, Antibody, and DNA Labeling: Enhances visibility for research, diagnostics, and imaging.
- Small Molecule Labeling: Performs well in organic solvents, suitable for various compounds.
- Instrument Compatibility: Works with fluorescent microscopes, scanners, and readers.
For delicate proteins, use the sulfo-Cyanine5 NHS ester, which avoids organic co-solvents while retaining similar fluorescent properties. Cyanine5 NHS Ester is a go-to reagent for bright, reliable labeling in scientific and diagnostic applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
