Terazine-activated Cy5 dye reacts with TCO-containing compounds via a Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.
Cy5 Tetrazine is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. Its absorption and emission spactra are almost identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
This is sulfonated dye is also known as sulfo-Cyanine5.
Cy®Dye is a trademark of GE Healthcare. Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
