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HomeFluorescent DyeCyanine DyesCyanine5 Dye Cy5 NHS ester
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Cy3 Maleimide $125.00 – $2,365.00
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Cy5 Maleimide $122.00 – $2,365.00
Cy5 NHS ester

Catalog #: AP12467
Name: Cy5 NHS ester
Molecular Weight: 739.86
Solubility: Water, DMSO, DMF
Abs/Em Maxima: 648/671 nm
Extinction coefficient: 250,000 cm-1M-1
Spectrally similar dyes: Alexa Fluor® 647, DyLight® 649,
Purity: ≥95%
Availability: In Stock

Cy5 NHS ester

$135.00 – $2,018.00

UnitPriceQuantity
1mg$135
5mg$348
25mg$910
100mg$2018

Would you like to inquire about bulk pricing?

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SKU: AP12467 Category: Cyanine5 Dye
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  • Description
  • Abs/Em Spectra
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Description

Cy5 dye (also sold under Sulfo-Cyanine5 name) is one of the most popular far-red fluorescent dyes. It is often a reagent of choice for protein and peptide labeling. Cy5 dye is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. Cy5 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. A significant advantage to using long wavelength dyes such as Cy5 or AF 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. We do not recommend using Cy5 NHS ester for labeling proteins at high molar ration due significant self-quenching. Cy5 conjugates are recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using APDye 647 (Alexa Fluor® 647 analog), or IR 650 dye (IRDye® 650 analog), which can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.

Abs/Em Spectra

Cy5

Shipping & Delivery
Ships within: 24 hours
Storage condition: -20 ° C
Shipping: Ambient Temperature

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