Cy5 Azide NHS Ester (CAS: 1564286-24-3) is a dual-functional fluorescent dye combining Cy5’s bright far-red fluorescence (~649/670 nm), an azide for click chemistry, and an NHS ester for amine coupling. It supports efficient biomolecule labeling for imaging and analytical research.
Key Features:
- Fluorescence:
- Excitation/Emission: ~649/670 nm (similar to Alexa Fluor® 647, CF® 647)
- Bright and Stable: Produces intense far-red fluorescence for high-sensitivity detection.
- Dual Reactivity:
- Azide Group: Enables click chemistry with alkynes or strained cyclooctynes.
- NHS Ester: Reacts with primary amines on proteins, peptides, and other biomolecules.
- Water-Soluble and pH-Stable:
- Soluble in water, DMSO, and DMF
- Fluorescence remains stable from pH 4 to 10
Applications:
- Microscopy: High-resolution imaging
- Flow Cytometry: Compatible with 633/647 nm lasers
- Bioconjugation: Protein, peptide, and biomolecule labeling
Storage:
- Form: Blue solid
- Conditions: Store at -20°C, protected from light and moisture
This product is an essential tool for advanced biological research, offering precise and versatile labeling for a wide range of applications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
