Cy5 Azide is a far-red fluorescent dye with absorption and emission spectra nearly identical to Alexa Fluor® 647, CF® 647 Dye, and other Cyanine5-based fluorescent dyes. It features an azide group that reacts with terminal alkynes through a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, enabling efficient biomolecule labeling. It also performs copper-free “click chemistry” with strained cyclooctynes, forming stable triazoles without requiring copper catalysts or high temperatures.
Key Properties:
- Absorption/Excitation: ~649-650 nm
- Emission: ~668-670 nm
- Laser Compatibility: Works well with 633 nm and 647 nm laser lines.
- pH Stability: Fluorescence remains stable in pH 4 to pH 10.
- Water Solubility: Fully soluble in water for biological applications.
Cy5 Azide Applications:
- Fluorescence Microscopy: High-resolution far-red imaging.
- Flow Cytometry: Compatible with standard laser lines.
- Bioconjugation: Labeling of biomolecules through click chemistry reactions.
- Copper-Free Reactions: Suitable for live-cell imaging and sensitive biological applications.
Cy5 Azide is an essential probe for advanced molecular labeling, imaging, and diagnostics, offering high brightness, water solubility, and chemical versatility.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
