Far-red fluorescent Cy5 azide absorption and emission spectra are nearly identical to those of Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5-based fluorescent dye, which reacts with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctynes to form stable triazoles via a copper-free “click chemistry” reaction and does not require copper catalysts or high temperatures. This far-red fluorescent probe is water-soluble and its fluorescence is pH-insensitive in the pH 4 to pH 10 range. Its excitation peak is well suited for 633 nm or 647 nm laser lines.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.