Calibro® Fluor 610 Carboxylic Acid is a non-reactive variant of the Calibro® Fluor 610 dye, designed primarily as a reference standard in fluorescence-based experiments. Known for its bright red-orange fluorescence, this xanthene fluorophore is highly stable and serves versatile roles in advanced biochemical applications.
Key Features:
- Reference Standard: Calibro® Fluor 610 Carboxylic Acid is used as a reliable reference dye in experiments involving Calibro® Fluor 610 conjugates, ensuring consistent spectral comparisons.
- Chemical Reactivity: The carboxylic acid group can react with hydrazines, hydroxylamines, and amines after activation by carbodiimides such as EDAC, allowing for further chemical modifications and custom conjugations.
- High Purity: The structure of Calibro® Fluor 610 Carboxylic Acid avoids the formation of multiple isomers during biomolecule conjugation, resulting in a single, well-defined peak in RP-HPLC and a clear emission spectrum.
- Stability: The dye’s stability through synthesis and processing stages makes it ideal for demanding applications, including qPCR, fluorescence microscopy, and flow cytometry.
- Compatibility: It can be used alongside fluorescence quenchers like DusQ 2, enhancing the sensitivity and specificity of qPCR and other detection assays.
Applications:
Calibro® Fluor 610 Carboxylic Acid is widely used in qPCR, molecular biology, and diagnostic assays, offering robust performance as a reference dye or in customized bioconjugation. Its reliable fluorescence and structural stability make it an essential tool for precise and high-sensitivity fluorescence applications.
The exceptional stability, purity, and versatility of Calibro® Fluor 610 Carboxylic Acid provide researchers with a dependable choice for fluorescence-based experiments and chemical modifications.
AxisPharm offers 5000+ PEG Linkers with high purity. Different kinds of PEG Reagents may be available by custom synthesis.
